高效液相色谱法检测五氟利多片的含量

Datetime:2024-07-12 From:

五氟利多片的含量测定

1. 参照依据:

2020版《中国药典》二部第80页下的五氟利多片含量检测,要求理论塔板数按五氟利多峰计算应不低于2000,五氟利多峰与相邻杂质峰的分离度应符合要求。

2. 实验结果

    器:S6000 UV;

柱:Alphasil VC-C18, 4.6 × 250 mm, 5 µm, 100Å;

相:A相:0.2%三乙胺溶液(用磷酸调节pH值至2.5);B相:甲醇

洗脱条件:A:B=30:70;

    速:1.0 mL/min;

    温:25 ℃;

量:10 μL;

检测波长:219 nm;


图1  五氟利多片含量测定色谱图 

(峰号:1,五氟利多)

表1  五氟利多片含量测定结果表

名称

保留时间(min

拖尾因子

USP理论塔板数

USP分离度

1

五氟利多

10.49

1.15

8007

5.92


相关仪器:
相关耗材:
Message form

Thank you for your attention and trust in Acchrom Tech products. If you have any questions or abnormal situations with the products during browsing the website, please leave a message or call our company.
Customer Service Hotline :400-108-7908, We will contact you as soon as possible and wish you and your family happiness and health!

Chromatographic Column Recommendation Assistant